Isolation of N-acetylneuraminic Acid and N-glycolylneuraminic Acid from Pleurocybella porrigens
نویسندگان
چکیده
منابع مشابه
N-glycolylneuraminic acid and N-acetylneuraminic acid define feline blood group A and B antigens.
Blood group incompatibility causes transfusion reactions and neonatal isoerythrolysis in cats. We investigated the molecular nature of the blood group antigens from cats that had blood type A, B, and AB erythrocytes. Naturally occurring anti-type B antibodies, Triticum vulgaris lectin, monoclonal antibody (MoAb) 32-27, and MoAb R-24 were used in agglutination tests, Western blots, and thin-laye...
متن کاملPreferential Accumulation of 14C-N-Glycolylneuraminic Acid over 14C-N-Acetylneuraminic Acid in the Rat Brain after Tail Vein Injection
The two main molecular species of sialic acid existing in nature are N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc). Neu5Ac is abundant in mammalian brains and plays crucial roles in many neural functions. In contrast, Neu5Gc is present only at a trace level in vertebrate brains. The brain-specific suppression of Neu5Gc synthesis, which is a common feature in mammals, s...
متن کاملCatalytic preference of Salmonella typhimurium LT2 sialidase for N-acetylneuraminic acid residues over N-glycolylneuraminic acid residues☆
In a comparison of sialidase activities toward N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc), we found that Salmonella typhimurium LT2 sialidase (STSA) hardly cleaved 4-methylumbelliferyl Neu5Gc (4MU-Neu5Gc). The k cat/K m value of STSA for 4MU-Neu5Gc was found to be 110 times lower than that for 4-methylumbelliferyl Neu5Ac (4MU-Neu5Ac). Additionally, STSA had remarkab...
متن کاملQuantification of N-acetylneuraminic Acid( Neu5ac) in Human Milk Using N-glycolylneuraminic Acid as Internal Standard by Lc-ms/ms
A rapid, reliable and sensitive LC-MS/MS method for the determination of Nacetylneuraminic acid (Neu5Ac) in human milk was developed and validated. Samples were hydrolysed with mild hydrochloric acid and analysed by LC-MS/MS on Thermo hypersilgold C-18 analytical column. The analytical procedure was found to be more selective, accurate, precise and linear. The method accuracy was 99.9 % and the...
متن کاملSynthesis of N-acetylneuraminic acid and of CMP-N-acetylneuraminic acid in the rat liver cell.
Adult male rats, under starving and normal conditions, were injected intravenously with N-acetyl[3H]mannosamine and after various time intervals the specific radioactivities of free N-acetylneuraminic acid (NeuAc) and CMP-N-acetylneuraminic acid were determined in the liver. The specific radioactivity of free NeuAc was high even within 20s after injection; the maximum was reached between 7 and ...
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ژورنال
عنوان ژورنال: Journal of Health Science
سال: 2009
ISSN: 1344-9702,1347-5207
DOI: 10.1248/jhs.55.373